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Studies of RISUG 1976 - 2005

Compiled and maintained by the Male Contraception Information Project.

Safety and toxicology studies 1981-2001

Verma K, Misro MM, Singh H, Mahajan S, Ray AR, Guha SK. "Histology of the rat vas deferens after injection of a non-occlusive chemical contraceptive." J Reprod Fertil. 1981 Nov; 63(2): 539-42. PMID: 7299756 [PubMed - indexed for MEDLINE]

Guha SK. "Electrical effects on mammalian sperm." In: Engineering in Medicine and Biology Society, 1988. Proceedings of the Annual International Conference of the IEEE. Pub. Date: 4-7 Nov 1988; vol.4 p 1881-1882.

Sethi N, Srivastava RK, Singh RK. "Safety evaluation of a male injectable antifertility agent, styrene maleic anhydride, in rats." Contraception. 1989 Feb; 39(2): 217-26. PMID: 2706990 [PubMed - indexed for MEDLINE]

Sethi N, Srivastava RK, Singh RK. "Histological changes in the vas deferens of rats after injection of a new male antifertility agent "SMA" and its reversibility." Contraception. 1990 Mar; 41(3): 333-9. PMID: 2323221 [PubMed - indexed for MEDLINE]

Sethi N, Srivastava RK, Singh RK. "Male mediated teratogenic potential evaluation of new antifertility compound SMA in rabbit (Oryctolagus cuniculus)." Contraception. 1990 Aug; 42(2): 215-23. PMID: 2085972 [PubMed - indexed for MEDLINE]

Sethi N, Srivastava RK, Singh RK, Bhatia GS, Sinha N. "Chronic toxicity of styrene maleic anhydride, a male contraceptive, in rhesus monkeys (Macaca mulatta)." Contraception. 1990 Sep; 42(3): 337-47. PMID: 2289393 [PubMed - indexed for MEDLINE]

Sethi N, Srivastava RK, Singh RK, Nath D. "Long-term toxicity studies of styrene maleic anhydride in rats." Biomed Environ Sci. 1990 Dec; 3(4): 452-7. PMID: 2096850 [PubMed - indexed for MEDLINE]

Sethi N, Srivastava RK, Nath D, Singh RK. "Preclinical toxicity study of a male injectable antifertility agent (styrene maleic anhydride) in rhesus monkeys, Macaca mulatta." J Med Primatol. 1991; 20(2): 89-93. PMID: 1865485 [PubMed - indexed for MEDLINE]

Sethi N, Srivastava RK, Nath D, Singh RK. "Teratological evaluation of an injectable male antifertility agent, styrene maleic anhydride, in rats." Int J Fertil. 1992 May-Jun; 37(3): 183-7. PMID: 1355766 [PubMed - indexed for MEDLINE]

Sharma U, Chaudhury K, Jagannathan NR, Guha SK. "A proton NMR study of the effect of a new intravasal injectable male contraceptive RISUG on seminal plasma metabolites." Reproduction. 2001 Sep; 122(3): 431-6. PMID: 11597307 [PubMed - indexed for MEDLINE]

Sharma U, Chaudhury K, Jagannathan NR, Guha SK. "A proton NMR study of the effect of a new intravasal injectable male contraceptive RISUG on seminal plasma metabolites." Reproduction. 2001 Sep; 122(3): 431-6. PMID: 11597307 [PubMed - in process]

Toxicology Study Report - Shriram Institute for Industrial Research - Acute Toxicity LD50 Rat (intraperitoneal) Unpublished; available from Prof. Sujoy K. Guha.

Assessment of Polystyrene (SMA) in Bead Form as a Bio-Implant. Unpublished; available from Prof. Sujoy K. Guha.

 

Feasibility, efficacy and reversibility studies 1976-2001

Guha, SK, Kaur H, Ahmed, AM. "Formulation of a fertilization index for male fertility studies." Med Life Sci Eng. 1975; 1: 17-27.

Guha SK, Kaur H, Ahmed AM. "Mechanics of spermatic fluid transport in the vas deferens." Med Biol Eng. 1975 Jul; 13(4): 518-22. No abstract available. PMID: 811934 [PubMed - indexed for MEDLINE]

Guha SK, Anand S. "Gamete transport mechanism." In: Talwar GP, editor. Recent advances in reproduction and regulation of fertility. Amsterdam: Elsevier; 1979. p 211-16.

Misro M, Guha SK, Singh H, Mahajan S, Ray AR, Vasudevan P. "Injectable non-occlusive chemical contraception in the male - I." Contraception. 1979 Nov; 20(5): 467-73. PMID: 43211 [PubMed - indexed for MEDLINE]

Singh H, Vasudevan P, Misro M, Ray AR, Guha SK. "Novel mode of contraception using polymeric hydrogels. I." J Biomed Mater Res. 1982 Jan; 16(1): 3-9. PMID: 7056760 [PubMed - indexed for MEDLINE]

Misro MM, Kaur H, Mahajan S, Guha SK. "An intravasal non-occlusive contraceptive device in rats." J Reprod Fertil. 1982 May; 65(1): 9-13. PMID: 7077606 [PubMed - indexed for MEDLINE]

Mahajan S, Guha SK, Misro MM. "A theoretical and scanning electron microscope study on the electrically mediated inactivation of spermatozoa." J Biol Phys. (Journal of Biological Physics) 1982; 10: 112/113-123/124.

Guha SK, Ansari S, Anand S, Farooq A, Misro MM, Sharma DN. "Contraception in male monkeys by intra-vas deferens injection of a pH lowering polymer." Contraception. 1985 Jul; 32(1): 109-18. PMID: 4053602 [PubMed - indexed for MEDLINE]

Guha SK, Anand S, Ansari S, Farooq A, Sharma DN. "Time-controlled injectable occlusion of the vas deferens." Contraception. 1990 Mar; 41(3): 323-31. PMID: 2323220 [PubMed - indexed for MEDLINE]

Guha SK. "Male fertility regulation by intra-vas deferens injection." In: Moudgal NR, Yoshinaga K, Rao AJ, Adiga PR, editors. Perspectives in primate reproductive biology. Bangalore: Wiley Eastern Limited; 1991. p 345-51.

Guha SK, Singh G, Anand S, Ansari S, Kumar S, Koul V. "Phase I clinical trial of an injectable contraceptive for the male." Contraception. 1993 Oct; 48(4): 367-75. PMID: 8222664 [PubMed - indexed for MEDLINE]

Koul V, Ansari S, Sharma K, Anand S, Guha SK. "Dose-dependent relationship of polymeric hydrogels on motility and vitality of human spermatozoa in vitro." Journal of Materials Science: Materials in Medicine. 1994 Apr; 6(4): 192-6.

Koshy LM. "Charging the vas deferens -- a novel male contraceptive method." (Review article) Indian Med Trib. 1994 Aug 30; 2(14): 6. PMID: 12179180 [PubMed - indexed for MEDLINE]

Guha SK. "Injectable contraceptive for the male." Expert Opin Investig Drugs. 1995 June; 4(6): 537 43.

Guha SK. "Contraceptive for use by a male." United States Patent 5488075; Jan. 30, 1996.

Guha SK, Singh G, Ansari S, Kumar S, Srivastava A, Koul V, Das HC, Malhotra RL, Das SK. "Phase II clinical trial of a vas deferens injectable contraceptive for the male." Contraception. 1997 Oct; 56(4): 245-50. PMID: 9408706 [PubMed - indexed for MEDLINE]

Lohiya NK, Manivannan B, Mishra PK. "Ultrastructural changes in the spermatozoa of langur monkeys Presbytis entellus entellus after vas occlusion with styrene maleic anhydride." Contraception. 1998 Feb; 57(2): 125-32. PMID: 9589840 [PubMed - indexed for MEDLINE]

Lohiya NK, Manivannan B, Mishra PK, Pathak N, Balasubramanian SP. "Intravasal contraception with styrene maleic anhydride and its noninvasive reversal in langur monkeys (Presbytis entellus entellus)." Contraception. 1998 Aug; 58(2): 119-28. PMID: 9773267 [PubMed - indexed for MEDLINE]

Guha SK, Singh G, Srivastava A, Das HC, Bhardwaj JC, Mathur V, Koul V, Malhotra RL and Das SK "Two-year clinical efficacy trial with dose variations of a vas deferens injectable contraceptive for the male." Contraception 1998; 58:165-174.

Koul V, Srivastava A, Guha SK. "Reversibility with sodium bicarbonate of styrene maleic anhydride, an intravasal injectable contraceptive, in male rats." Contraception. 1998 Oct; 58(4): 227-31. PMID: 9866004 [PubMed - indexed for MEDLINE]

Guha SK. "Current status of development and clinical testing of male intravasal contraceptive." In: Rajalakshmi M, Griffin PD, editors. Male contraception: present and future. New Delhi: New Age International; 1998/1999. p 274-85.

Manivannan B, Mishra PK, Lohiya NK. "Ultrastructural changes in the vas deferens of langur monkeys Presbytis entellus entellus after vas occlusion with styrene maleic anhydride and after its reversal." Contraception. 1999 Feb; 59(2): 137-44. PMID: 10361629 [PubMed - indexed for MEDLINE]

Guha SK. "Non-invasive reversal of intraluminal vas deferens polymer injection-induced azoospermia--technology." Asian J Androl. 1999 Sep; 1(3): 131-4. PMID: 11250780 [PubMed - indexed for MEDLINE]

Lohiya NK, Manivannan B, Mishra PK. "Repeated vas occlusion and non-invasive reversal with styrene maleic anhydride for male contraception in langur monkeys." Int J Androl. 2000 Feb; 23(1): 36-42. PMID: 10632760 [PubMed - indexed for MEDLINE]

Lohiya NK, Manivannan B, Mishra PK, Pathak N. "Vas deferens, a site of male contraception: an overview." Asian J Androl. 2001 Jun; 3(2): 87-95. PMID: 11404791 [PubMed - in process]

Sharma RS, Rajalakshmi M, Sharma RS, Jeyaraj DA. "Current status of fertility control methods in India." J Biosci. 2001 Nov; 26(4 Suppl): 391-405. Review. PMID: 11779954 [PubMed - indexed for MEDLINE]

Lohiya NK, Manivannan B, Mishra PK, Pathak N. "Non-invasive reversible vas occlusion with styrene maleic anhydride: a possible alternative to vasectomy." In: Puri CP, Van Look PFA, editors. Sexual and reproductive health: recent advances, future directions. New Delhi: New Age Int (P) Ltd; 2001. p 249-68.

Recent studies 2001-2005

Chaudhury K, Sharma U, Jagannathan NR, Guha SK. "Effect of a new injectable male contraceptive on the seminal plasma amino acids studied by proton NMR spectroscopy." Contraception. 2002 Sep; 66(3): 199-204. PMID: 12384210 [PubMed - indexed for MEDLINE]

Singh AK. "Intra-vas deferens injection of styrene maleic anhydride gel for male contraception: is it safe?" J Fam Plann Reprod Health Care. 2002 Oct; 28(4): 208-9. No abstract available. PMID: 12419065 [PubMed - indexed for MEDLINE]

Chaube SK, Misro MM. "Effect of styrene maleic anhydride (SMA) on viability and integrity of isolated rat oocytes in vitro." Contraception. 2002 Dec; 66(6): 469-72. PMID: 12499041 [PubMed - in process]

Chaki SP, Das HC, Misro MM. "A short-term evaluation of semen and accessory sex gland function in phase III trial subjects receiving intravasal contraceptive RISUG." Contraception. 2003 Jan; 67(1): 73-8. PMID: 12521662 [PubMed - indexed for MEDLINE]

Mishra PK, Manivannan B, Pathak N, Sriram S, Bhande SS, Panneerdoss S, Lohiya NK. "Status of Spermatogenesis and Sperm Parameters in Langur Monkeys Following Long-term Vas Occlusion With Styrene Maleic Anhydride." J Androl. 2003 Jul-Aug; 24(4): 501-9. PMID: 12826690 [PubMed - indexed for MEDLINE]

Sharma S, Sen P, Mukhopadhyay SN, Guha SK. "Microbicidal male contraceptive-Risug induced morphostructural damage in E. coli." Colloids Surf B Biointerfaces. 2003 Oct; 32(1): 43-50.

Chaudhury K, Bhattacharyya AK, Guha SK. "Studies on the membrane integrity of human sperm treated with a new injectable male contraceptive." Hum Reprod. 2004 Aug; 19(8): 1826-30. Epub 2004 Jun 10. PMID: 15192063 [PubMed - in process]

Guha SK. "RISUG™ (Reversible Inhibition of Sperm Under Guidance) - An  antimicrobial as male vas deferens implant for HIV free semen." Med Hypotheses. 2005;65(1):61-4. PMID: 15893119 [PubMed - indexed for MEDLINE]

Lohiya NK, Manivannan B, Mishra PK, Sriram S, Bhande SS, Panneerdoss S. "Preclinical evaluation for noninvasive reversal following long-term vas occlusion with styrene maleic anhydride in langur monkeys." Contraception. 2005 Mar;71(3):214-26. PMID: 15722073 [PubMed - indexed for MEDLINE]

Manivannan B, Bhande SS, Panneerdoss S, Sriram S, Lohiya NK. "Safety evaluation of long-term vas occlusion with styrene maleic anhydride and its non-invasive reversal on accessory reproductive organs in langurs." Asian J Androl. 2005 Jun;7(2):195-204. PMID: 15897977 [PubMed - indexed for MEDLINE]

Kumar S, Chaudhury K, Sen P, Guha SK. "Atomic force microscopy: a powerful tool for high-resolution imaging of spermatozoa." J Nanobiotechnology. 2005 Sep 27;3:9. PMID: 16188038 [PubMed - indexed for MEDLINE]

DMSO Resources: Toxicology reviews

Ali BH. "Dimethyl sulfoxide: recent pharmacological and toxicological research." Vet Hum Toxicol. 2001 Aug; 43(4): 228-31. Review. PMID: 11474739 [PubMed - indexed for MEDLINE]

Santos NC, Figueira-Coelho J, Martins-Silva J, Saldanha C. "Multidisciplinary utilization of dimethyl sulfoxide: pharmacological, cellular, and molecular aspects." Biochem Pharmacol. 2003 Apr 1; 65(7): 1035-41. Review. PMID: 12663039 [PubMed - indexed for MEDLINE]

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